1
Browse > Jason Moffat > Mak et al > pLD-puro-PGKnVA
Plasmid 24589: pLD-puro-PGKnVA

  • Gateway(TM) cassette

  • 1705

  • VA

  • N terminal on insert

  • pLKO.1
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • Gateway Cloning

  • attR1

  • Yes

  • attR2

  • Yes

  • mPGK-F List of Sequencing Primers

  • Ampicillin

  • ccdB Survival

  • 30

  • DB3.1 or ccdB resistance strain. We do not allow E. coli harbouring this plasmid to grow to saturation (no longer than 14 hours). We prefer to grow it at 30C and 180RPM of shaking when applicable.

  • Low Copy

  • Puromycin

  • View sequences (1)

  • Jason Moffat

  • MTA

Comments: 

The mouse PGK promoter was PCR-amplified and cloned into pLD-puro-CnVA (Plasmid #24587) to generate pLD-puro-PGKnVA.

VA= versatile affinity tag, 3XFlag, 6XHis+StrepIII

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A lentiviral-based functional proteomics approach identifies chromatin remodelling complexes important for the induction of pluripotency. Mak et al (Mol Cell Proteomics. 2010 Mar 19. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24589" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only