OKSIM
(Plasmid #24603)

• Depositing Lab: Jose Cibelli
• Publication: Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

Backbone

• Vector backbone: pSin4-EF2-IRES-Pur
• Backbone size w/o insert (bp): 6777
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Oct4 KLF4 Sox2 c-Myc
• Species: H. sapiens (human)
• Insert Size (bp): 5600
• Mutation: Oct4-2A-KLF4-2A-Sox2-IRES-Myc
• Entrez Gene: SOX2 (a.k.a. ANOP3, MCOPS3)
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Mlu I (not destroyed)
• 3′ cloning site: Pme I (not destroyed)
• 5′ sequencing primer: EF-1a Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The pSin4-EF2-IRES-Pur was obtained from Addgene (James Thomson's laboratory) in the form of pSin-EF2-Oct4-Pur. The WPRE/LoxP LTR portion was obtained from Addgene in the form of pWPT-GFP (Luigi Naldini's laboratory). Transgene ORFs were cloned from human factor cDNAs.
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by Steven T. Suhr, MSU Cellular Reprogramming Laboratory