Addgene - pAR3-Lux Plasmid Data

Plasmid 24643: pAR3-Lux

Gene/insert name:
None
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Vector backbone:
pGL2-basic
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size (bp):
5597
Modifications to Backbone:
3ARE + TATA box
Cloning site 5':
EcoRI
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
Yes
5' sequencing primer:
LucNRev List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Jeff Wrana
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Took insert containing 3ARE + TATA box from A3-CAT using blunted HindIII and XhoI into pGL2-basic with a blunted EcoRI and XhoI. ARE sequence - TATCTGCTGCCCTAAAATGTGTATTCCATGGAAATGTCTGCCCTTCTCTC

NB: The A30-CAT map is attached, NOT the map for this plasmid (AR3-Lux)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24643" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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