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EF-hGPC3
(Plasmid #24670)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24670 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPC3
  • Species
    H. sapiens (human)
  • Entrez Gene
    GPC3 (a.k.a. DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer EF-1a Forward
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

HA tag is after leader sequence. HA tag sequence is - PVYDVPDYA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF-hGPC3 was a gift from Jorge Filmus (Addgene plasmid # 24670 ; http://n2t.net/addgene:24670 ; RRID:Addgene_24670)
  • For your References section:

    OCI-5/GPC3, a glypican encoded by a gene that is mutated in the Simpson-Golabi-Behmel overgrowth syndrome, induces apoptosis in a cell line-specific manner. Gonzalez AD, Kaya M, Shi W, Song H, Testa JR, Penn LZ, Filmus J. J Cell Biol. 1998 Jun 15. 141(6):1407-14. 10.1083/jcb.141.6.1407 PubMed 9628896