Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24670||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneGPC3 (a.k.a. DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer EF-1a Forward (Common Sequencing Primers)
HA tag is after leader sequence. HA tag sequence is - PVYDVPDYA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EF-hGPC3 was a gift from Jorge Filmus (Addgene plasmid # 24670 ; http://n2t.net/addgene:24670 ; RRID:Addgene_24670)
For your References section:OCI-5/GPC3, a glypican encoded by a gene that is mutated in the Simpson-Golabi-Behmel overgrowth syndrome, induces apoptosis in a cell line-specific manner. Gonzalez AD, Kaya M, Shi W, Song H, Testa JR, Penn LZ, Filmus J. J Cell Biol. 1998 Jun 15. 141(6):1407-14. 10.1083/jcb.141.6.1407 PubMed 9628896