Addgene - mPERK Plasmid Data

Plasmid 24826: mPERK

Gene/insert name:
mPERK
Alt name:
Eif2ak3
Species:
M. musculus (mouse)
Entrez Gene:
Eif2ak3 (AI427929, Pek, Perk)
Vector backbone:
pLenti CMV/TO Puro DEST (670-1)
(Search Vector Database)
Backbone manufacturer:
Eric Campeau (Addgene plasmid 17293)
Vector type:
Mammalian Expression, Lentiviral ; Destination vector
Backbone size w/o insert (bp):
9594
Cloning method:
Gateway Cloning
Cloning site 5':
Gateway
Site destroyed during cloning:
Unknown
Cloning site 3':
Gateway
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
MSCV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Fumihiko Urano
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Transcriptional regulation of VEGF-A by the unfolded protein response pathway. Ghosh et al (PLoS One. 2010 . 5(3):e9575. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24826" in your Materials and Methods section.