Addgene - human p53-Δ(325-355) Plasmid Data

Plasmid 24871: human p53-Δ(325-355)

Gene/insert name:
p53
Insert size:
1089
Species:
H. sapiens (human)
Entrez Gene:
TP53 (BCC7, LFS1, P53, TRP53)
Fusion protein or tag:
His
Terminal:
N terminal on insert
Mutation:
Deletion of aa 325-355
Vector backbone:
pET15b
(Search Vector Database)
Backbone manufacturer:
Novagen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5700
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

E.Coli strain for epression: BL21DE3-pLysS

p53 proteins are expressed better and more stable at low temperature

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Latent and active p53 are identical in conformation. Ayed et al (Nat Struct Biol. 2001 Sep . 8(9):756-60. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24871" in your Materials and Methods section.