This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

mATF4
(Plasmid #24874)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24874 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti CMV/TO Puro DEST (670-1)
  • Backbone manufacturer
    Eric Campeau (Addgene plasmid 17293)
  • Backbone size w/o insert (bp) 9594
  • Vector type
    Mammalian Expression, Lentiviral ; Destination vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse ATF4
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_009716
  • Entrez Gene
    Atf4 (a.k.a. Atf-4, C/ATF, CREB2, TAXREB67)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (unknown if destroyed)
  • 3′ cloning site Gateway (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer MSCV-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mATF4 was a gift from Fumihiko Urano (Addgene plasmid # 24874)
  • For your References section:

    Transcriptional regulation of VEGF-A by the unfolded protein response pathway. Ghosh R, Lipson KL, Sargent KE, Mercurio AM, Hunt JS, Ron D, Urano F. PLoS One. 2010 . 5(3):e9575. 10.1371/journal.pone.0009575 PubMed 20221394