Gal4-HA-HIF1α(530-652)
(Plasmid #24887)

• Depositing Lab: William Kaelin
• Publication: Ivan et al Science. 2001 Apr 20. 292(5516):464-8.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5252
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha or similar
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hypoxia inducible factor1alpha
• Alt name: Gal4-HA-HIF-ODD
• Alt name: oxygen-dependent degradation domain
• Species: H. sapiens (human)
• Insert Size (bp): 909
• Mutation: partial HIF1alpha cDNA (encoding aa 530-652)fused to Gal4 DNA binding domain, fused to HA-tag
• Entrez Gene: HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
• Tags / Fusion Proteins:
  • Gal4dbd (N terminal on backbone)
  • HA-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: CMV-forw or T7
• 3′ sequencing primer: Sp6

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry