pcDNA3-HA+WT GADD45
(Plasmid #24929)

• Depositing Lab: Xin Wang
• Publication: Yang et al J Biol Chem. 2000 Nov 24. 275(47):36892-8.

Backbone

• Vector backbone: pcDNA3-HA
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GADD45
• Species: H. sapiens (human)
• Insert Size (bp): 495
• Entrez Gene: GADD45A (a.k.a. DDIT1, GADD45)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid size: 5867 bp

The HA sequence is between XbaI and ApaI restriction enzyme sites.