Addgene - shRNA-DNMT1i(CDS) Plasmid Data

Plasmid 24950: shRNA-DNMT1i(CDS)

Gene/insert name:
shRNA DNMT1i CDS
Species:
H. sapiens (human)
Vector backbone:
pSuper Retro
(Search Vector Database)
Backbone manufacturer:
OligoEngine
Vector type:
Mammalian Expression, Retroviral, RNAi
Backbone size w/o insert (bp):
6349
5' sequencing primer:
pLXSN-5 List of Sequencing Primers
3' sequencing primer:
H1
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Paul Khavari
Terms and Licenses:
MTA

Comments:

DNMT1i shRNA oligo seq is - GGAACCAAGCAAGAAGTGA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: DNMT1 maintains progenitor function in self-renewing somatic tissue. Sen et al (Nature. 2010 Jan 28. 463(7280):563-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24950" in your Materials and Methods section.