Addgene - U6-shRNA pgkCre Plasmid Data

Plasmid 24971: U6-shRNA pgkCre

Gene/insert name:
shRNA
Insert size:
22
Vector backbone:
unknown
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7705
Cloning site 5':
HpaI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
MSCV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

This vector allows for constitutive Cre and shRNA expression. It does not contain any selection cassette. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/jacks-lab/protocols/pSico.html

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Tissue-specific p19Arf regulation dictates the response to oncogenic K-ras. Young et al (Proc Natl Acad Sci U S A. 2010 Jun 1. 107(22):10184-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24971" in your Materials and Methods section.