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pCFJ66
(Plasmid #24981)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2544
  • Vector type
    Multisite Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    cb-unc-119 Punc-122::GFP
  • Species
    C. elegans (nematode); C. briggsae
  • Insert Size (bp)
    4300

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attB1 (destroyed during cloning)
  • 3′ cloning site attB2R (destroyed during cloning)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ66 was a gift from Erik Jorgensen (Addgene plasmid # 24981)
  • For your References section:

    Targeted gene deletions in C. elegans using transposon excision. Frokjær-Jensen C, Davis MW, Hollopeter G, Taylor J, Harris TW, Nix P, Lofgren R, Prestgard-Duke M, Bastiani M, Moerman DG, Jorgensen EM. Nat Methods. 2010 Apr 25. ():. 10.1038/nmeth.1454 PubMed 20418868