pT7-EGFP-C1-HsTNRC6A
(Plasmid #25035)

• Depositing Lab: Elisa Izaurralde
• Publication: Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4770
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Dh5 alpha or XL1-blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TNRC6A
• Species: H. sapiens (human)
• Insert Size (bp): 5130
• GenBank ID: NM_014494
• Entrez Gene: TNRC6A (a.k.a. CAGH26, FAME6, GW1, GW182, TNRC6)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry