Addgene - pBabe-puro-miR-10b sponge Plasmid Data

Plasmid 25043: pBabe-puro-miR-10b sponge

Gene/insert name:
miR-10b sponge
Insert size:
186
Vector backbone:
pBabe-puro
(Search Vector Database)
Backbone manufacturer:
Bob Weinberg, Addgene plasmid 1764
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
XhoI
Site destroyed during cloning:
Unknown
Cloning site 3':
ApaI
Site destroyed during cloning:
Unknown
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

8 tandem binding sites to human miR-10b. Repeated sequence is CACAAATTCGGAGAACAGGGTA.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Therapeutic silencing of miR-10b inhibits metastasis in a mouse mammary tumor model. Ma et al (Nat Biotechnol. 2010 Apr . 28(4):341-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25043" in your Materials and Methods section.