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pJT103
(Plasmid #25087)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25087 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSB4A3
  • Backbone manufacturer
    BioBrick vector-Shetty et al 2008
  • Backbone size w/o insert (bp) 3473
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PompC-lacZ
  • Alt name
    light transfer function measuring construct
  • Species
    E.coli
  • Insert Size (bp)
    3075

Cloning Information

  • Cloning method Restriction Enzyme
  • 3′ cloning site Spe1 (unknown if destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT103 was a gift from Christopher Voigt (Addgene plasmid # 25087 ; http://n2t.net/addgene:25087 ; RRID:Addgene_25087)
  • For your References section:

    A synthetic genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Cell. 2009 Jun 26. 137(7):1272-81. 10.1016/j.cell.2009.04.048 PubMed 19563759