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pBabe-Flag hMcl-1
(Plasmid #25371)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25371 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe IRES puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • GenBank ID
    NM_021960
  • Entrez Gene
    MCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site blunt (destroyed during cloning)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer pBabe 5'
  • 3′ sequencing primer pBabe 3'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

There are a few mismatches between the sequence provided and the existing NCBI sequence.
The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.

Addgene NGS did not identify IRES sequence in plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-Flag hMcl-1 was a gift from Roger Davis (Addgene plasmid # 25371 ; http://n2t.net/addgene:25371 ; RRID:Addgene_25371)
  • For your References section:

    Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Morel C, Carlson SM, White FM, Davis RJ. Mol Cell Biol. 2009 Jul . 29(14):3845-52. 10.1128/MCB.00279-09 PubMed 19433446