Addgene - pBabe-Flag hMcl-1 Plasmid Data

Plasmid 25371: pBabe-Flag hMcl-1

Gene/insert name:
MCL1
Insert size:
1100
Species:
H. sapiens (human)
GenBank ID:
NM_021960
Entrez Gene:
MCL1 (BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pBabe IRES puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
blunt
Site destroyed during cloning:
Yes
Cloning site 3':
Sal I
Site destroyed during cloning:
No
5' sequencing primer:
pBabe 5' List of Sequencing Primers
3' sequencing primer:
pBabe 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Roger Davis
Terms and Licenses:
MTA

Comments:

There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D173E mutation.
The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Morel et al (Mol Cell Biol. 2009 Jul . 29(14):3845-52. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25371" in your Materials and Methods section.