Addgene - pCDNA3.1-hMcl-1(S159A) Plasmid Data

Plasmid 25374: pCDNA3.1-hMcl-1(S159A)

Gene/insert name:
hMCL1(S159A)
Alt name:
MCL1
Insert size:
1100
Species:
H. sapiens (human)
GenBank ID:
NM_021960
Entrez Gene:
MCL1 (BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
Fusion protein or tag:
V5 and His tag
Terminal:
C terminal on backbone
Mutation:
changed serine 159 to alanine
Vector backbone:
pcDNA3.1D V5-His-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5514
Cloning method:
TOPO Cloning
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Ulrich Maurer-"Glycogen Synthase Kinase-3 Regulates Mitochondrial Outer Membrane Permeabilization and Apoptosis by Destabilization of MCL-1"
Sequence: View sequences (3)
Principal Investigator:
Roger Davis
Terms and Licenses:
MTA

Comments:

There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D172N D173E mutations.
The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.

The tags on this plasmid are not in frame with hMcl-1.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Morel et al (Mol Cell Biol. 2009 Jul . 29(14):3845-52. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25374" in your Materials and Methods section.