|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25375||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1D V5-His-TOPO
- Backbone size w/o insert (bp) 5514
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1100
Entrez GeneMCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
/ Fusion Protein
- V5 and His tag (C terminal on backbone)
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer n/a (Common Sequencing Primers)
There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D173E mutation.
The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.
The tags on this plasmid are not in frame with hMcl-1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA3.1-hMcl-1 was a gift from Roger Davis (Addgene plasmid # 25375 ; http://n2t.net/addgene:25375 ; RRID:Addgene_25375)
For your References section:Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Morel C, Carlson SM, White FM, Davis RJ. Mol Cell Biol. 2009 Jul . 29(14):3845-52. 10.1128/MCB.00279-09 PubMed 19433446