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pCDNA3.1-hMcl-1(T163A)
(Plasmid #25376)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3.1D V5-His-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5514
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    hMCL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • Mutation
    changed threonine 163 to alanine
  • GenBank ID
    NM_021960
  • Tag / Fusion Protein
    • V5 and His tag (C terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D173E mutation.
The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.

The tags on this plasmid are not in frame with hMcl-1.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1-hMcl-1(T163A) was a gift from Roger Davis (Addgene plasmid # 25376)
  • For your References section:

    Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Morel C, Carlson SM, White FM, Davis RJ. Mol Cell Biol. 2009 Jul . 29(14):3845-52. 10.1128/MCB.00279-09 PubMed 19433446