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Plasmid 25376: pCDNA3.1-hMcl-1(T163A)
  • hMCL1

  • 1100

  • H. sapiens (human)

  • NM_021960

  • V5 and His tag

  • C terminal on backbone

  • changed threonine 163 to alanine

  • pcDNA3.1D V5-His-TOPO
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression

  • 5514

  • TOPO Cloning

  • T7 List of Sequencing Primers

  • n/a

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Neomycin

  • View sequences (3)
  • Roger Davis

  • UBMTA

Comments: 

There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D173E mutation.
The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.

The tags on this plasmid are not in frame with hMcl-1.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Morel et al (Mol Cell Biol. 2009 Jul . 29(14):3845-52. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25376" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only