Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

F3/-2.5CAT
(Plasmid #25425)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25425 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCATdeltaEH
  • Backbone manufacturer
    Mellon Lab
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression ; Reporter construct

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myo D Promoter + F3
  • Alt name
    MyoD
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYOD1 (a.k.a. CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1)
  • Tag / Fusion Protein
    • CAT (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer CAT-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fragment 3 was cloned into the XbaI site of -2.5CAT by digesting chMD-13 with NotI and EcoRI, and blunt-end-ligating the fragment into the unique XbaI site.

The -2.5 promoter fragment can be removed by digestion with SalI or NotI and XhoI. The resulting fragment is approximately 6.8kb.

All tk sequences were removed from the CAT vector in the construction of -2.5kb.

Between the enhancer and promoter is a portion of pBluescript polylinker that includes BamHI, SpeI and SmaI sites, none of which are unique.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    F3/-2.5CAT was a gift from Charles P. Emerson Jr. (Addgene plasmid # 25425 ; http://n2t.net/addgene:25425 ; RRID:Addgene_25425)
  • For your References section:

    Regulatory elements that control the lineage-specific expression of myoD. Goldhamer DJ, Faerman A, Shani M, Emerson CP. Science. 1992 Apr 24. 256(5056):538-42. 10.1126/science.1315077 PubMed 1315077