|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25433||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7072
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
Gene/Insert nameDEST cassette from pcDNADEST40
Insert Size (bp)1900
MutationpQCXI retroviral backbone, in which Eric Campeau replaced the antibiotic resistance gene with DsRed2 fluorescent protein. Next, the Gateway DEST cassette from pcDNADEST40 was inserted into the Age I site. This cassette includes the original V5, His tags at C-terminal end, and both can be used to tag proteins
/ Fusion Protein
- V5 (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ cloning site Age I (not destroyed)
- 3′ cloning site Age I (not destroyed)
- 5′ sequencing primer LNCX (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe plasmid pQCXIR was received from Eric Campeau. The DEST cassette was cloned into the plasmid in our lab by by Octavian Henegariu, M.D.
Terms and Licenses
Any mismatches between Addgene QC sequenced and the depositing lab's sequence is inconsequential.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQCXIR-DEST was a gift from Kevan Herold (Addgene plasmid # 25433)