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pQCXIR-DEST
(Plasmid #25433)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25433 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQCXI
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7072
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DEST cassette from pcDNADEST40
  • Insert Size (bp)
    1900
  • Mutation
    pQCXI retroviral backbone, in which Eric Campeau replaced the antibiotic resistance gene with DsRed2 fluorescent protein. Next, the Gateway DEST cassette from pcDNADEST40 was inserted into the Age I site. This cassette includes the original V5, His tags at C-terminal end, and both can be used to tag proteins
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Age I (not destroyed)
  • 3′ cloning site Age I (not destroyed)
  • 5′ sequencing primer LNCX
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid pQCXIR was received from Eric Campeau. The DEST cassette was cloned into the plasmid in our lab by by Octavian Henegariu, M.D.
  • Terms and Licenses

Depositor Comments

Any mismatches between Addgene QC sequenced and the depositing lab's sequence is inconsequential.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIR-DEST was a gift from Kevan Herold (Addgene plasmid # 25433)