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pHS12-mCherry
(Plasmid #25444)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25444 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHS12
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry
  • Species
    Discosoma sp.
  • Insert Size (bp)
    711
  • GenBank ID
    AY678264
  • Tag / Fusion Protein
    • COX4 Presequence (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Roger Tsien, UCSD.
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is derived from a construct created by Rob Jensen's group. It encodes mitochondrially-targeted mCherry expressed from the constitutive ADH1 promoter. Cells containing this plasmid have bright red mitochondria with apparently normal morphology.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS12-mCherry was a gift from Benjamin Glick (Addgene plasmid # 25444 ; http://n2t.net/addgene:25444 ; RRID:Addgene_25444)