pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc
(Plasmid #25458)

• Depositing Lab: Betty Eipper
• Publication: Chufan et al Structure. 2009 Jul 15. 17(7):965-73.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5472
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PAM
• Alt name: peptidyl-alpha-hydroxyglycine alpha-amidating lyase
• Species: R. norvegicus (rat)
• Insert Size (bp): 966
• Mutation: Contains aa 498-820 (PALcc= PAL catalytic core) Serine 767 mutated to Alanine (delta Glyc)
• Entrez Gene: Pam (a.k.a. PHM)
• Tags / Fusion Proteins:
  • PAM signal (N terminal on insert)
  • His6 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: T7, CMV-F
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry