Addgene - Actin-BAF53(a+b)-ires-EGFP Plasmid Data

Plasmid 25461: Actin-BAF53(a+b)-ires-EGFP

Gene/insert name:
BAF53(a+b)
Insert size:
1474
Species:
M. musculus (mouse)
Fusion protein or tag:
EGFP
Terminal:
C terminal on backbone
Mutation:
3' of mBAF53a deleted and fused to 3' of mBAF53b
Vector backbone:
Beta-actin/CMV-IRES-EGFP
(Search Vector Database)
Backbone manufacturer:
home made
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6000
Cloning site 5':
EcoR1
Site destroyed during cloning:
No
Cloning site 3':
EcoR1
Site destroyed during cloning:
No
5' sequencing primer:
gttattgtgctgtctcatc List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Jerry Crabtree
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of dendritic development by neuron-specific chromatin remodeling complexes. Wu et al (Neuron. 2007 Oct 4. 56(1):94-108. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25461" in your Materials and Methods section.