Addgene - HUW-TetO-hNanog Plasmid Data

Plasmid 25700: HUW-TetO-hNanog

Gene/insert name:
Nanog
Insert size:
926
Species:
H. sapiens (human)
Entrez Gene:
NANOG ()
Vector backbone:
FUW-TetO
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
8377
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
AAAGTGAAAGTCGAGCTCGGTACC List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Direct cell reprogramming is a stochastic process amenable to acceleration. Hanna et al (Nature. 2009 Dec 3. 462(7273):595-601. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25700" in your Materials and Methods section.