pGEX-EGFAB(H306Y)
(Plasmid #25771)

• Depositing Lab: Johann Deisenhofer
• Publication: McNutt et al J Biol Chem. 2009 Apr 17. 284(16):10561-70.

Backbone

• Vector backbone: pGEX
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LDL receptor EGFAB
• Species: H. sapiens (human)
• Insert Size (bp): 240
• Mutation: His 306 to Tyr
• Entrez Gene: LDLR (a.k.a. FH, FHC, FHCL1, LDLCQ2)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NCO1 (not destroyed)
• 3′ cloning site: ECOR1 (not destroyed)
• 5′ sequencing primer: pGEX 5'
• 3′ sequencing primer: pGEX 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated in the Deisenhofer lab but first described in J Biol Chem. 2009 Apr 17. 284(16):10561-70.