TU#867 (Punc-119sid-1)
(Plasmid #25811)

• Depositing Lab: Martin Chalfie
• Publication: Calixto et al Nat Methods. 2010 May 30. ():.

Backbone

• Vector backbone: pPD95.75
• Backbone manufacturer: A. Fire
• Backbone size w/o insert (bp): 4000
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: systemic RNA interference-deficient 1
• Alt name: sid-1
• Species: C. elegans (nematode)
• Insert Size (bp): 7860
• Entrez Gene: sid-1 (a.k.a. CELE_C04F5.1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: PvuI (not destroyed)
• 5′ sequencing primer: TATAGGATCCTCATTTTTCCAGGTTCACAATG
• 3′ sequencing primer: ATATAAGCGGCCGCAGAAAGGTGTCATGGTCTAGT

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Specific details about this vector are given at the Materials and Methods of the article: Calixto et al, 2010, Nat Meth "Enhanced Neuronal RNAi using sid-1"

Note that this plasmid contains genomic sid-1, NOT the cDNA.