Addgene - pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase Plasmid Data

Plasmid 25846: pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase

Gene/insert name:
LplA-W37I
Alt name:
Alternative coumarin PRIME ligase
Alt name:
Less sensitive, more specific
Insert size:
1042
Species:
E. Coli
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Mutation:
W37I
Vector backbone:
pcDNA3
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Original LplA gene a gift from John Cronan (Univ. of Illinois)
Sequence: View sequences (2)
Principal Investigator:
Alice Ting
Terms and Licenses:
MTA

Comments:

The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A fluorophore ligase for site-specific protein labeling inside living cells. Uttamapinant et al (Proc Natl Acad Sci U S A. 2010 Jun 15. 107(24):10914-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25846" in your Materials and Methods section.