|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25894||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2790
Vector typeGateway Entry vector
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1653
- Cloning method Gateway Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
This is a control entry vector. There is no Stop codon.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDONR223_Luciferase was a gift from David Root (Addgene plasmid # 25894 ; http://n2t.net/addgene:25894 ; RRID:Addgene_25894)
For your References section:A public genome-scale lentiviral expression library of human ORFs. Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE. Nat Methods. 2011 Jun 26. ():. 10.1038/nmeth.1638 PubMed 21706014