Addgene - pNANOG-Luc Plasmid Data

Plasmid 25900: pNANOG-Luc

Gene/insert name:
NANOG promoter
Insert size:
404
Species:
H. sapiens (human)
GenBank ID:
NT_086792
Entrez Gene:
NANOG ()
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Mutation:
None
Vector backbone:
pGL4.10
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4242
Cloning site 5':
Xho I
Site destroyed during cloning:
No
Cloning site 3':
Bgl II
Site destroyed during cloning:
No
5' sequencing primer:
RVprimer3 List of Sequencing Primers
3' sequencing primer:
Ref to Promega
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Ren-he Xu
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: NANOG is a direct target of TGFbeta/activin-mediated SMAD signaling in human ESCs. Xu et al (Cell Stem Cell. 2008 Aug 7. 3(2):196-206. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25900" in your Materials and Methods section.