Addgene - pSIN4-EF2-ABCG2-IRES-Neo Plasmid Data

Plasmid 25983: pSIN4-EF2-ABCG2-IRES-Neo

Gene/insert name:
ATP-binding cassette, sub-family G (WHITE), member 2
Alt name:
ABCG2
Insert size:
1968
Species:
H. sapiens (human)
GenBank ID:
NM_004827
Entrez Gene:
ABCG2 (ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, EST157481, GOUT1, MRX, MXR, MXR1, UAQTL1)
Fusion protein or tag:
EF1-apha promoter
Terminal:
N terminal on backbone
Fusion protein or tag:
IRES-Neo
Terminal:
C terminal on backbone
Mutation:
None
Vector backbone:
pSIN-EF2-MCS-IRES-Neo
(Search Vector Database)
Backbone manufacturer:
James Thomson
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
6500
Cloning site 5':
EcoR I
Site destroyed during cloning:
Unknown
Cloning site 3':
BamH I
Site destroyed during cloning:
Unknown
5' sequencing primer:
EF-1a-F List of Sequencing Primers
3' sequencing primer:
IRES-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Ren-he Xu
Terms and Licenses:
MTA

Comments:

Please note that the backbone of this plasmid is larger than the reported 6.5kb (see attached diagnostic digest).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lack of ABCG2 expression and side population properties in human pluripotent stem cells. Zeng et al (Stem Cells. 2009 Oct . 27(10):2435-45. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25983" in your Materials and Methods section.