pSIN4-EF2-ABCG2-IRES-Neo
(Plasmid #25983)

• Depositing Lab: Ren-he Xu
• Publication: Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

Backbone

• Vector backbone: pSIN-EF2-MCS-IRES-Neo
• Backbone manufacturer: James Thomson
• Backbone size w/o insert (bp): 6500
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2
• Alt name: ABCG2
• Species: H. sapiens (human)
• Insert Size (bp): 1968
• Mutation: None
• GenBank ID: AAH92408.1
• Entrez Gene: ABCG2 (a.k.a. ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, CDw388, EST157481, GOUT1, MRX, MXR, MXR-1, MXR1, UAQTL1)
• Tags / Fusion Proteins:
  • EF1-apha promoter (N terminal on backbone)
  • IRES-Neo (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR I (unknown if destroyed)
• 3′ cloning site: BamH I (unknown if destroyed)
• 5′ sequencing primer: EF-1a-F
• 3′ sequencing primer: IRES-R

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the backbone of this plasmid is larger than the reported 6.5kb (see attached diagnostic digest).