Addgene - 458 p5E-uasE1b Plasmid Data

Plasmid 26026: 458 p5E-uasE1b

Gene/insert name:
E1B basal promoter + UAS repeats
Insert size:
522
Vector backbone:
pENTR
(Search Vector Database)
Vector type:
Entry vector
Backbone size w/o insert (bp):
2605
Cloning method:
Gateway Cloning
Cloning site 5':
attL1
Site destroyed during cloning:
No
Cloning site 3':
attR2
Site destroyed during cloning:
No
5' sequencing primer:
M13pUC-fwd List of Sequencing Primers
3' sequencing primer:
M13rev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Nathan Lawson
Terms and Licenses:
MTA

Comments:

"p5E" refers to 5' entry clones flanked by L4-R1.

There are 2 mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches do not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26026" in your Materials and Methods section.