|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26026||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2605
Vector typeEntry vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameE1B basal promoter + UAS repeats
Insert Size (bp)522
- Cloning method Gateway Cloning
- 5′ cloning site attL1 (not destroyed)
- 3′ cloning site attR2 (not destroyed)
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Terms and Licenses
"p5E" refers to 5' entry clones flanked by L4-R1.
There are 2 mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:458 p5E-uasE1b was a gift from Nathan Lawson (Addgene plasmid # 26026)
Generated by Addgene from full sequence supplied by depositor.