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ChR2-YFP-NavII-III
(Plasmid #26057)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26057 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE
  • Backbone manufacturer
    Addgene plasmid 20945
  • Backbone size w/o insert (bp) 10093
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NavII-III
  • Alt name
    sodium channel subunit Nav1.2
  • Alt name
    sodium channel, voltage-gated
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    684
  • Tag / Fusion Protein
    • EYFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (unknown if destroyed)
  • 3′ cloning site BsrGI (unknown if destroyed)
  • 5′ sequencing primer CMV-F, EGFP-C
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ChR2-YFP-NavII-III was a gift from Matthew Grubb (Addgene plasmid # 26057)
  • For your References section:

    Channelrhodopsin-2 localised to the axon initial segment. Grubb MS, Burrone J. PLoS One. 2010 . 5(10):e13761. 10.1371/journal.pone.0013761 PubMed 21048938