Addgene - pBTBX-3 Plasmid Data

Plasmid 26069: pBTBX-3

Gene/insert name:
None
Vector backbone:
pBTBX-3
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size (bp):
3677
Cloning site 5':
NheI
Site destroyed during cloning:
Unknown
Cloning site 3':
HincII
Site destroyed during cloning:
Unknown
5' sequencing primer:
atacccgttt ttttgggcta List of Sequencing Primers
Bacterial resistance(s)
Chloramphenicol
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Ryan Gill
Terms and Licenses:
MTA

Comments:

There is an extra G in the middle of the alignment between Addgene's quality control sequence and the author's sequence. This discrepancy is in a non-coding region and does affect function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Broad-host-range vectors for protein expression across gram negative hosts. Prior et al (Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26069" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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