pSVE Grb2 myc 49L
(Plasmid #26085)

• Depositing Lab: Bob Weinberg
• Publication: Egan et al Nature. 1993 May 6. 363(6424):45-51.

Backbone

• Vector backbone: pSVE
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Grb2 P49L
• Alt name: Grb2
• Species: H. sapiens (human)
• Insert Size (bp): 650
• Mutation: P49L
• Entrez Gene: GRB2 (a.k.a. ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2)
• Tag / Fusion Protein:
  • myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBABE-3
• 3′ sequencing primer: M13 reverse?

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mutagenesis performed using oligo GGA AAA GAC GGC TTC ATT TTA AAG AAC TAC ATA GAA ATG, which creates a new DraI site and mutates codon 49 from P to L.