Addgene - pBabe-puro-mIR-31 Plasmid Data

Plasmid 26088: pBabe-puro-mIR-31

Gene/insert name:
miR-31
Alt name:
miR31
Insert size:
400
Species:
H. sapiens (human)
Entrez Gene:
MIR31 (MIRN31, hsa-mir-31, miR-31)
Vector backbone:
pBABE-puro
(Search Vector Database)
Backbone manufacturer:
Weinberg lab (Addgene#:1764)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
SalI
Site destroyed during cloning:
Unknown
5' sequencing primer:
pBabe-5 List of Sequencing Primers
3' sequencing primer:
pBabe-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

The human miR-31 gene was PCR-amplified from genomic DNA and cloned into
pcDNA3.1 (Invitrogen), then subcloned into the BamHI and SalI sites of the pBABE-puro
retroviral vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. Valastyan et al (Cell. 2009 Jun 12. 137(6):1032-46. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26088" in your Materials and Methods section.