Backbone manufacturerStructural Genomics Consortium
- Backbone size (bp) 7328
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- Hexahistidine tag with Thrombin cleavage (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28a-LIC was a gift from Cheryl Arrowsmith (Addgene plasmid # 26094)
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.