23SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U
(Plasmid
#26191)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26191 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonemodified pUC19
- Backbone size w/o insert (bp) 2876
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name23S ribosomal RNA fragment (modified) with G2553U mutation
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Alt name23S rRNA with G2553U mutation
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Insert Size (bp)74
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Mutationfragment 2508-2580 of E. coli rRNA, with nucleotides 2530-2534 replaced with GAAAC and an rA 3' to nucleotide 2580 plus G2553U mutation
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NgoMIV (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Lac promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
23SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U was a gift from David McKay (Addgene plasmid # 26191 ; http://n2t.net/addgene:26191 ; RRID:Addgene_26191) -
For your References section:
Structure of the RNA Binding Domain of a DEAD-Box Helicase Bound to Its Ribosomal RNA Target Reveals a Novel Mode of Recognition by an RNA Recognition Motif. Hardin JW, Hu YX, McKay DB. J Mol Biol. 2010 Jul 29. ():. 10.1016/j.jmb.2010.07.040 PubMed 20673833
