Addgene - pXL-BACII-attPGAL4LWL Plasmid Data

Plasmid 26260: pXL-BACII-attPGAL4LWL

Gene/insert name:
GAL4
Insert size:
2965
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
GAL4 (YPL248C, GAL81)
Vector backbone:
pXL-BacII-ECFP
(Search Vector Database)
Vector type:
Bacterial Expression, Insect Expression ; Drosophila transgenesis
Backbone size w/o insert (bp):
9110
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SacI
Site destroyed during cloning:
No
5' sequencing primer:
CAGTGCACGTTTGCTTGTTGA List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pGaTB (obtained from Drosophila Genomics Resource Center)
Sequence: View sequences (5)
Map:
View map
Principal Investigator:
Tom Clandinin
Terms and Licenses:
MTA

Comments:

Brand and Perrimon. Development. 1993 Jun;118(2):401-15.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile in vivo system for directed dissection of gene expression patterns Gohl et al (Nature Methods (2011) doi:10.1038/nmeth.1561)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26260" in your Materials and Methods section.