Addgene - pBRPyCAG-fmKlf4-DsRed-Ip Plasmid Data

Plasmid 26274: pBRPyCAG-fmKlf4-DsRed-Ip

Gene/insert name:
Klf4
Insert size:
1450
Species:
M. musculus (mouse)
Entrez Gene:
Klf4 (RP23-322L22.2, EZF, Gklf, Zie)
Vector backbone:
pBRYCAG-floxStop dsRED-IresPuro
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
8050
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
gtccccttctccctctccagcctcgg List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs. Hanna et al (Proc Natl Acad Sci U S A. 2010 May 18. 107(20):9222-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26274" in your Materials and Methods section.