Addgene - pQCXIG (w497-1) Plasmid Data

Plasmid 26286: pQCXIG (w497-1)

Gene/insert name:
no insert
Alt name:
Enhanced Green Fluorescent Protein
Vector backbone:
pQCXIP
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Retroviral
Backbone size (bp):
7116
Cloning site 5':
Not I
Site destroyed during cloning:
No
Cloning site 3':
Eco RI
Site destroyed during cloning:
No
5' sequencing primer:
CMVforw List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC.
High or low copy:
High Copy
Selectable markers, other:
GFP
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

A cDNA or miRNA can be cloned downstream of the CMV promoter. There is an IRES sequence followed by the GFP cDNA so transduced cells will be fluorescent.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26286" in your Materials and Methods section.