Plasmid 26303: pBCN28-R4R3

• Gene/insert name: None
• Vector backbone: pCG150
  (Search Vector Database)
• Vector type: Worm Expression
• Backbone size (bp): 8090
• Cloning site 5': AttR4
• Site destroyed during cloning: Yes
• Cloning site 3': AttR3
• Site destroyed during cloning: Yes
• 5' sequencing primer: M13 Reverse
• 3' sequencing primer: M13 (-21) Forward
• Bacterial resistance(s) Ampicillin and Chloramphenicol
• Growth strain(s) DB3.1
• Growth temperature (℃): 37
• Growth instructions: Use DB3.1 E. coli (must be ccdB resistant). Note that ccdB Survival bacteria (Invitrogen) do not work for this plasmid.
• High or low copy: Low Copy
• Selectable markers: Puromycin
• Person or lab that originally
  cloned the gene/insert: Puromycin resistance gene from pBabePuro (Addgene). rpl-28 promoter and let-858 3'UTR sequences from pPD129.57 vector (Addgene). mCherry gene from pGH8 (Addgene).
• Sequence: View sequences (3)
• Map: View map
• Principal Investigator: Ben Lehner
• Terms and Licenses: MTA

Comments: 

Use for puromycin selection of transgenes in Caenorhabditis species other than C. elegan (works also in C. elegans).
This vector is the same as pBCN22-R4R3 but with a different Pmyo-2::mCherry::unc-54_3'UTR construct that gives good fluoresence expression in the pharynx of C. elegans as well as other species, e.g. C. briggsae, C. remanei and C. brenneri.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Article: Rapid selection of transgenic C. elegans using antibiotic resistance. Semple et al (Nat Methods. 2010 Aug 22. ():. PubMed)