pMSCV-mLin28A
(Plasmid #26357)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pMSCV
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    lin-28 homolog A
  • Alt name
    lin28-a
  • Alt name
    lin28
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    630
  • Entrez Gene
    Lin28a (a.k.a. RP23-314D24.1, AL024421, ENSMUSG00000070700, Gm10299, Lin-28, Lin28, Tex17, lin-28A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pLXSN-5
  • 3′ sequencing primer MSCV-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-mLin28A was a gift from George Daley (Addgene plasmid # 26357)
  • For your References section:

    Lin28 promotes transformation and is associated with advanced human malignancies. Viswanathan SR, Powers JT, Einhorn W, Hoshida Y, Ng TL, Toffanin S, O'Sullivan M, Lu J, Phillips LA, Lockhart VL, Shah SP, Tanwar PS, Mermel CH, Beroukhim R, Azam M, Teixeira J, Meyerson M, Hughes TP, Llovet JM, Radich J, Mullighan CG, Golub TR, Sorensen PH, Daley GQ. Nat Genet. 2009 Jul . 41(7):843-8. 10.1038/ng.392 PubMed 19483683