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pDEST_EWSR1
(Plasmid #26377)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26377 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFRT_DESTFLAGHA
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 5299
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EWSR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1971
  • GenBank ID
    NM_005243
  • Entrez Gene
    EWSR1 (a.k.a. EWS, EWS-FLI1, bK984G1.4)
  • Tag / Fusion Protein
    • FLAG/HA (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST_EWSR1 was a gift from Thomas Tuschl (Addgene plasmid # 26377 ; http://n2t.net/addgene:26377 ; RRID:Addgene_26377)
  • For your References section:

    RNA targets of wild-type and mutant FET family proteins. Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, Hafner M, Borkhardt A, Sander C, Tuschl T. Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163. 10.1038/nsmb.2163 PubMed 22081015