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pDEST_TAF15
(Plasmid #26379)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pFRT_DESTFLAGHA
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 5299
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    TAF15
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1779
  • GenBank ID
    NM_139215
  • Entrez Gene
    TAF15 (a.k.a. Npl3, RBP56, TAF2N, TAFII68)
  • Tag / Fusion Protein
    • FLAG/HA (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST_TAF15 was a gift from Thomas Tuschl (Addgene plasmid # 26379)
  • For your References section:

    RNA targets of wild-type and mutant FET family proteins. Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, Hafner M, Borkhardt A, Sander C, Tuschl T. Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163. 10.1038/nsmb.2163 PubMed 22081015