pECE
(Plasmid #26453)

• Purpose: (Empty Backbone)
• Depositing Lab: William Rutter
• Publication: Ellis et al Cell. 1986 Jun 6;45(5):721-32.

Backbone

• Vector backbone: pECE
• Backbone manufacturer: Bill Rutter
• Backbone size (bp): 2924
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Empty vector
• Insert Size (bp): 2924

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: SV40pro-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid kindly provided to Addgene by Dr. Anne Brunet.

There are a few single nucleotide differences and one small insertion relative to author sequence but they do not alter the MCS or affect plasmid function.