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Plasmid 26478: pJC87.2-npy-6
  • npy-6

  • 264

  • S. mediterranea (planarian)

  • BK007031

  • pJC53.2
    (Search Vector Database)

  • T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

  • 3240

  • Eam1105I

  • Yes

  • Eam1105I

  • Yes

  • SP6 List of Sequencing Primers

  • T3

  • Ampicillin and Kanamycin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (3)
  • Phillip Newmark

  • UBMTA

Comments: 

dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Genome-wide analyses reveal a role for peptide hormones in planarian germline development. Collins et al (PLoS Biol. 2010 . 8(10):e1000509. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26478" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with