Addgene - MG Plasmid Data

Plasmid 26528: MG

Gene/insert name:
None
Fusion protein or tag:
eGFP
Terminal:
N terminal on backbone
Vector backbone:
pMIG-w
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid 12282
Vector type:
Mammalian Expression, Retroviral
Backbone size (bp):
6500
5' sequencing primer:
pLXSN-5 List of Sequencing Primers
3' sequencing primer:
MSCV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
David Baltimore
Terms and Licenses:
MTA

Comments:

MG is derived from MG155 (see plasmid 26529). MG was created by removing miR-155 with Not1 and Xho1, filling in the ends, and ligating the plasmid back together.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Sustained expression of microRNA-155 in hematopoietic stem cells causes a myeloproliferative disorder. O'Connell et al (J Exp Med. 2008 Mar 17. 205(3):585-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26528" in your Materials and Methods section.