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MG
(Plasmid #26528)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26528 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMIG-w
  • Backbone manufacturer
    Addgene plasmid 12282
  • Backbone size (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MG is derived from MG155 (see plasmid 26529). MG was created by removing miR-155 with Not1 and Xho1, filling in the ends, and ligating the plasmid back together.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MG was a gift from David Baltimore (Addgene plasmid # 26528 ; http://n2t.net/addgene:26528 ; RRID:Addgene_26528)
  • For your References section:

    Sustained expression of microRNA-155 in hematopoietic stem cells causes a myeloproliferative disorder. O'Connell RM, Rao DS, Chaudhuri AA, Boldin MP, Taganov KD, Nicoll J, Paquette RL, Baltimore D. J Exp Med. 2008 Mar 17. 205(3):585-94. 10.1084/jem.20072108 PubMed 18299402