Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]
(Plasmid #26568)

• Depositing Lab: Diane Lipscombe
• Publication: Pan et al J Neurosci. 2000 Jul 1. 20(13):4769-75.

Backbone

• Vector backbone: pcDNA6/V5-His ABC
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10F'
• Growth instructions: Top10F'
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: calcium channel alpha-1B subunit
• Species: R. norvegicus (rat)
• Insert Size (bp): 7390
• Mutation: contains exons a10, 18a, 31a, 37b, and 46. Deletion of exon 24a
• GenBank ID: AF055477
• Entrez Gene: Cacna1b (a.k.a. BIII)
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • 6XHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcorV (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Isolated from superior cervical ganglia.

For more information about this plasmid: http://neuroscience.brown.edu/LipscombeLab/homepage/otherpages/clonespage/clonespage1.htm

Also see: GENBANK AF222337