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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26575 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1/Hygro
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5600
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10F'
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Growth instructionsTOP10F'
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCacna2d1
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Alt namevoltage-gated calcium channel alpha2/delta-1 subunit
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SpeciesR. norvegicus (rat)
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Insert Size (bp)3965
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Entrez GeneCacna2d1 (a.k.a. CCHLA2, Cacna2, DHSCCA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site ApaI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GENBANK #AF286488
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CaVα2δ1 was a gift from Diane Lipscombe (Addgene plasmid # 26575 ; http://n2t.net/addgene:26575 ; RRID:Addgene_26575) -
For your References section:
Alternative splicing in the voltage-sensing region of N-Type CaV2.2 channels modulates channel kinetics. Lin Y, McDonough SI, Lipscombe D. J Neurophysiol. 2004 Nov . 92(5):2820-30. 10.1152/jn.00048.2004 PubMed 15201306
Map uploaded by the depositor.
